Biotechnology: Principles and Processes

In gel electrophoresis, separated DNA fragments
can be visualized with the help of

The sequence that controls the copy number of
the linked DNA in the vector, is termed

Identify the wrong statement with regard to
Restriction Enzymes

Choose the correct pair from the following

The specific palindromic sequence which is
recognized by EcoRl is

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the
incorrect statement.

DNA precipitation out of a mixture of bimolecules can be achieved by treatment with :

The correct order of steps in Polymerase Chain
Reaction (PCR) is

A gene whose expression helps to identify transformed cell is known as

DNA fragments are

The process of separation and purification of expressed protein before marketing is called :

The DNA fragments separated on an agarose gel can be visualised after staining with

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

Which of the following restriction enzymes produces blunt ends ?

Stirred-tank bioreactors have been designed for -

Which of the following is not a component of downstream processing ?

The taq polymerase enzyme is obtained from

Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

Which of the following is a restriction endonuclease ?

Which of the following is not a feature of the plasmids ?

The cutting of DNA at specific locations
became possible with the discovery of -

The DNA molecule to which the gene of
interest is integrated for cloning is called -

Commonly used vectors for human genome sequencing are :

Which vector can clone only a small fragment of DNA ?

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

During the process of isolation of DNA, chilled
ethanol is added to :

The colonies of recombinat bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of :

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

Which of the following is not corretly matched for the organism and its cell wall degrading enzyme ?

For transformation, micro - particles coated with DNA to be bombarded with gene are made up of

PCR and Restriction Fragment Length Polymorphism are the methods for

A single strand of nucleic acid tagged with a radioactive molecule is called

Which one is a true statement regarding DNA polymerase used in PCR ?

The figure below is the diagrammatic respesentation o the E.Coli vector pBR 322. Which one of the
given options correctly identifies its certain component(s) ?

Biolistics (gene-gun) is suitable for -

Which one of the following represents a palindromic sequence in DNA ?

In genetic engineering, the antibiotics are used

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents ?

There is a restriction endonuclease called
EcoRl. What does "co" part in it stand for ?

Agarose extracted from sea weeds finds use in :

Given below is a sample of a portion of DNA
strand giving the base sequence on the opposite
strands. What is so special shown in it ?
5' .............. GAATTC ................ 3'
3' .............. CTTAAG ................. 5'

Which one of the following techniques made it
possible to genetically engineer living organisms ?

Stirred-tank bioreactors have been designed
for –

Restriction endonucleases are enzymes which –

DNA or RNA segment tagged with a radioactive
moleculer is called –

Which one of the following is used as vector for
cloning genes into higher organisms ?

Which of the following are used in gene
cloning ?

In genetic engineering, a DNA segment (gene) of
interest,is transferred to the host cell through a
vector. Consider the following four agents (A-D)
in this regard and select the correct option about
which one or more of these can be used as...

Which one of the following is commonly used in
transfer of foreign DNA into crop plants ?