Biotechnology: Principles and Processes PYQs - Last 10 Years

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Practice 63 NEET Biology questions from Biotechnology: Principles and Processes. Use the year-wise and type-wise breakdown to prioritize recent PYQs, then continue into the question list below.

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Last 10 Years Biotechnology: Principles and Processes Questions

Showing 50 of 63 filtered questions.

1Biotechnology Principles And Processes

During PCR, primers bind to the DNA strands in the $\_\_\_\_$ step.

2Biotechnology Principles And Processes

Given below are two statements :
Statement I : Plasmids are autonomously replicating DNA.
Statement II: Plasmids are extrachromosomal DNA.
In the light of the above statements, choose the most appropriate answer from the options given below...

3Biotechnology Principles And Processes

\(\text { Match List-I with List-II. }\)

List-I

List-II

A.

Transformation

I.

Restriction enzyme

B.

Cloning site

II.

Transfer DNA to host bacteria

C.

Selection

III.

Replication

D.

Ori

IV.

Antibiot...

4Biotechnology Principles And Processes

Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R.
Assertion A : In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells.
Reason R...

5Biotechnology Principles And Processes

Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR322 results in the loss of antibiotic resistance towards:

6Biotechnology Principles And Processes

Which of the following statements are correct with respect to DNA separation, isolation and visualization?
A. The cutting of DNA is done by molecular scissors.
B. The DNA fragments separate according to their size in an agarose gel, upon el...

7Biotechnology Principles And Processes

Which of the following statements are not true regarding restriction endonucleases?
A. They are called molecular scissors.
B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli.
C. They cut the DNA onl...

8Biotechnology Principles And Processes

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction :

9Biotechnology Principles And Processes

\(\text { Match List I with List II: }\)

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10Biotechnology Principles And Processes

Which of the following enzyme(s) are NOT essential for gene cloning?
A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:

11Biotechnology Principles And Processes

Given below are two statements:
Statement 1 : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II : Smaller size DNA fragments are observed near anode while larger fragments are ...

12Biotechnology Principles And Processes

In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

13Biotechnology Principles And Processes

Identify the part of a bio-reactor which is used as a foam braker from the given figure.

14Biotechnology Principles And Processes

The blue and white selectable markers have been developed which differentiate recombinant colonies from nonrecombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Given below are two ...

15Biotechnology Principles And Processes

Silencing of specific mRNA is possible via RNAi because of

16Biotechnology Principles And Processes

Polymerase chain reaction (PCR) amplifies DNA following the equation.

17Biotechnology Principles And Processes

Following are the steps involved in the process of PCR.
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the...

18Biotechnology Principles And Processes

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tet\(^{\mathrm{R}}\)) gene in the pBR322 cloning vector.

19Biotechnology Principles And Processes

The Bt toxin in genetically engineered Bt cotton kills the pest by:

20Biotechnology Principles And Processes

Match List-I with List - II :

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21Biotechnology Principles And Processes

Identify the incorrect statement related to gel electrophoresis.

22Biotechnology Principles And Processes

Which of the following are correct about EcoRI?
A. Cut the DNA with blunt end
B. Cut the DNA with sticky end
C. Recognise a specific palindromic sequence
D. Cut the DNA between the base \(\mathrm{G}\) and \(\mathrm{A}\) when encounters the ...

23Biotechnology Principles And Processes

Given below are two statements:
Statement I : The Indian Government has set up GEAC, which will make decisions regarding the validity of GM research.
Statement II : Biopiracy is the term used to refer to the use of bio-resources by native p...

24Biotechnology Principles And Processes

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA
respectively with:

25Biotechnology Principles And Processes

The "Ti plasmid" of Agrobacterium tumefaciens stands for

26Biotechnology Principles And Processes

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

27Biotechnology Principles And Processes

Match List I with List II.

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28Biotechnology Principles And Processes

Ligation of foreign DNA at which of the following site will result in loss of tetracyclin resistance of pBR322 :

29Biotechnology Principles And Processes

Thermostable DNA polymerase used in PCR was isolated from :

30Biotechnology Principles And Processes

Which of the following is not a cloning vector?

31Biotechnology Principles And Processes

Refer to the following statements for agarose-gel electrophoresis:
(a) Agarose is a natural polymer obtained from sea-weed.
(b) The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA.
(c) The DNA migrates ...

32Biotechnology Principles And Processes

Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?

33Biotechnology Principles And Processes

Pathogenic bacteria gain resistance to antibiotics due to changes in their :

34Biotechnology Principles And Processes

Match List-I with List-II :

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35Biotechnology Principles And Processes

The enzyme (a) is needed for isolating genetic material from plant cells and enzyme (b) for isolating genetic material from fungus. Choose the correct pair of options from the following :

36Biotechnology Principles And Processes

Separation of DNA fragments is done by a technique known as

37Biotechnology Principles And Processes

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : When a particular restriction enzyme cuts strand of DNA, overhanging stretches or sticky ends are formed.
Reason (R) :...

38Biotechnology Principles And Processes

Which of the following is not a desirable feature of a cloning vector?

39Biotechnology Principles And Processes

Given below are two statements:
Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence.
Statement II : Restriction endonucleases cut the DNA strand a little away from the cent...

40Biotechnology Principles And Processes

Which one of the following statement is not true regarding gel electrophoresis technique?

41Biotechnology Principles And Processes

Given below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : Polymerase chain reaction is used in DNA amplification.
Reason (R) : The ampicillin resistant gene is used as a selec...

42Biotechnology Principles And Processes

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :

43Biotechnology Principles And Processes

During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

44Biotechnology Principles And Processes

Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for \(\beta\)-galactoside production and the recombinant plasmid is inserted in an E.coli stra...

45Biotechnology Principles And Processes

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

46Biotechnology Principles And Processes

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

47Biotechnology Principles And Processes

Match List-I with List-II .
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48Biotechnology Principles And Processes

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out :

49Biotechnology Principles And Processes

Match the organism with its use in biotechnology

(a) Bacillus thuringiensis
(i) Cloning vector

(b) Thermus aquaticus
(ii) Construction of first rDNA molecule

(c) Agrobacterium tumefaciens
(iii) DNA ...

50Biotechnology Principles And Processes

Bt cotton variety that was developed by
the introduction of toxin gene of Bacillus
thuringiensis (Bt) is resistant to