Biotechnology Principles And Processes

The replication fork is formed and DNA ligase activity of the Taq polymerase joins the discontinuous fragments.

The replication fork is formed and Taq polymerase extends DNA in both $3^{\prime}$ to $5^{\prime}$ and $5^{\prime}$ to $3^{\prime}$ direction.

Denaturation step in PCR substitutes for the replication fork and Taq polymerase extends DNA only in the $3^{\prime}$ to $5^{\prime}$ direction.

Denaturation step in PCR substitutes for the replication fork and Taq polymerase extends DNA only in the $5^{\prime}$ to $3^{\prime}$ direction.